United States securities and exchange commission logo June 17, 2024 Edward Kaye Chief Executive Officer Stoke Therapeutics, Inc. 45 Wiggins Avenue Bedford, MA 01730 Re: Stoke Therapeutics, Inc. Registration Statement on Form S-3 Filed June 13, 2024 File No. 333-280172 Dear Edward Kaye: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jason Drory at 202-551-8342 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Julia Forbess